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KMID : 1094720160210060777
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Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 6 p.777 ~ p.786
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Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation
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Luo Miao-Miao
Ren Lu-Jing
Chen Sheng-Lan
Ji Xiao-Jun
Huang He
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Abstract
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Vitamin K2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.
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KEYWORD
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Bacillus natto, menaquinone-7, extraction, optimization, sporulation ratio
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